Harmonised population-based surveys with recent HIV-1 infection testing algorithms permit pooled cross-sectional estimation of HIV incidence across multiple countries. We aimed to estimate adult HIV-1 incidence rates and number of new infections by sex, age, and subregion in sub-Saharan Africa. The authors analysed data from 13 Population-Based HIV Impact Assessment (PHIA) surveys and two additional population-based surveys done between 2015 and 2019 in 15 sub-Saharan African countries. HIV- seropositive samples from adults aged 15-59 years were tested for recent HIV-1 infection by use of an algorithm consisting of the HIV-1 limiting antigen avidity enzyme immunoassay, HIV-1 viral load, and qualitative detection of antiretroviral agents. Data were pooled across countries; sampling weights were incorporated to represent all adults in the 15 national populations. Analyses accounted for the complex sample designs. HIV incidence rates, incidence rate differences, and number of new annual infections were estimated. Among 445-979 adults sampled, 382 had recent HIV-1 infection. The estimated HIV-1 incidence rate was 3-3 per 1000 person-years (95% CI 26-40) among women and 20 per 1000 person-years (12-27) among men (incidence rate difference 1-3 per 1000 person-years, 95% CI 03-23). Among adults aged 15-24 years, the incidence rate was higher for women (3-5 per 1000 person-years) than men (12 per 1000 person-years; difference 2-3, 95% CI 08-38), but infection rates were similar between sexes in all other age groups. The HIV-1 incidence rate was 7-4 per 1000 person-years (95% CI 50-97) in southern sub-Saharan Africa, 2-3 per 1000 person-years (17-29) in the eastern subregion, and 0-9 per 1000 person-years (06-12) in the western and central subregion. 689-000 (95% CI 546000-83300) new HIV cases were estimated annually among the 265 million susceptible adults (61-6% in women).